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One of the most interesting applications of the improved uranium lead zircon technique has to do with its ability to achieve nearly concordant results from single grains extracted from sandstone.
We analyzed 125 grains extracted from 35 hand specimens collected from weathering profiles at 11 sites located at 3 distinct elevations.
The experiments also indicated that wolf pruning at 5% produced a proper count of the protruding grains extracted from grinding wheel surface.
We report on material-property studies and OSL-dating results for polymineral fine grains extracted from piston cores obtained from the northern basin of Lake Malawi during the 1998 campaign of the International Decade of the East African Lakes (IDEAL).
The occurrence of pollen dimorphism in the three lines was studied by measuring the diameter of the pollen grains extracted from a single anther of each of the 100 male cyathia, 3.2 mm in diameter.
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The bioreactor medium contained 60 g/l lactose with 40% (v/v) of the same spent grain extract.
The QM6a Δ mus53 with the 83 kb deletion in the scaffold 15 (Del scaff15) and the parental strain were cultivated in Trichoderma minimal medium (TrMM) [ 24] supplemented with lactose and spent grain extract (see materials and methods).
Bioreactor cultivations for transcriptional profiling were performed in triplicate from a single inoculum per strain in one litre Sartorius Q DCU4 bioreactors using 4% lactose and 2% spent grain extract; all other parameters were controlled as in the previous batch method.
To study cellulase production, the deletion strains and the QM6a Δmustrainrain were cultivated in shake flasks in TrMM supplemented with 4% lactose, 2% spent grain extract 0.05% peptone pH 4.8 for 7 days at 28°C.
Similar to the results in TrMM lactose spent grain extract medium, there was no significant difference in growth or in cellulase production between Del scaff15 and the parent strain (data not shown).
T. reesei QM9414 and representative clones from transformations of each of the regulatory factor constructs were cultivated on medium containing 4% lactose (Fagron), 2% spent grain extract, 7.6 g/l (NH4 2SO4, 15.0 g/l KH2PO4, 2.4 mM MgSO4.7H2O, 4.1 mM CaCI2.H2O, 3.7 mg/l CoCI2, 5 mg/l FeSO4.7H2O, 1.4 mg/l ZnSO4.7H2O and 1.6 mg/l MnSO4.7H2O, pH adjusted to 5.2 with KOH.
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