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Images of the graft sections were acquired using a Zeiss fluorescence axial microscope attached with a digital camera.
The paraffin-embedded fat graft sections were incubated with the antibodies overnight at room temperature followed by incubation with appropriate secondary antibodies [31].
Microvascular density (MVD) in fat grafts was determined in five regions of interest where the CD31 antibody signal was the most intense in each section in all of the fat graft sections.
Quantification of macrophage infiltration in the fat grafts was estimated by counting the number of CD68-positive cells in five fields per fat graft in all fat graft sections.
Graft sections were mounted on super frost/plus glass, as described above, and deparaffinized.
Representative islet graft sections from a vehicle control and an alogliptin-treated mouse engrafted with islets from Donor 1 are shown in Figure 5A and B, respectively.
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Chamber construction is described in more detail in the top graft section.
To prove their theory, researchers grafted sections of human ovaries into mice and then exposed them to the hydrocarbons.
The grafts section worked on rules introduced in the Book of Vile Darkness and the Fiend Folio, adding new undead "grafts", but did not include rules for creating your own.
To detect apoptotic cells in xenogeneic graft tumors, sections were analyzed by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end-labeling (TUNEL) (Roche Molecular Biochemicals), according to the manufacturer's instructions.
To make up for the lack of an overture and musical connecting tissue, Mr. Sellars has grafted in sections from another early Mozart opera, "Thamos, King of Egypt".
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