Cells induced with RA for 12 hr and subsequently recultured in liquid culture gradually expressed the differentiated phenotype and lost transferrin receptor expression.
When cultured, these cells gradually expressed ASMA, as well as vimentin, smoothelin and desmin.
Although ALP is not an osteogenic-specific gene, it is always gradually expressed in the early period of osteogenic differentiation and declined in late period [ 28].
When inducer was added, enzyme synthesis resumed, suggesting that the transferred i + gene was becoming gradually expressed and the zygote became phenotypically inducible (fig. 5).
In this study, we demonstrated that the detoxification and metabolism processes are gradually expressed in H. arsenicoxydans to adapt to arsenic-rich environments.
Our interest first focused on the epithelial mucin, MUC4, in this disease because it is not expressed by normal pancreatic tissue but is gradually expressed during pancreatic carcinogenesis from the premalignant lesions, that is, pancreatic intraepithelial neoplasia (PanIN) type 1 (which are MUC4-positive in 17% of the cases) to PDAC (Swartz et al, 2002).
Microarray assays revealed that the cells gradually started to express osteogenic genes, whereas expression of GC-specific genes decreased.
He fostered then a unity, a hard core of reliable competitors, some of whom he gradually allowed to express their individuality.
It is of note that DC-SIGN expression gradually decreased without expressing Langerin when 3-day-LC-like cells were washed to remove TGF-β1 and further incubated with complete culture medium (CCM) plus GM-CSF (Fig. 3D).
After NeuroD1 is turned on the progenitors leave the cell cycle, gradually mature, express PSA-NCAM, NeuroD2, Calretinin, Prox1, Tbr1 and, finally, NeuN.
