Pellets were washed three times in cacodylate buffer, treated with 1% osmium for 1 h 30 min at room temperature, washed three times in water and incubated for 1 h with 2% uranyl acetate (w/v) in 25% ethanol before grading series of dehydration in ethanol.
After washing the cells in 0.05 mol L-1 maleate, the cells were dehydrated in a grading series of ethanol followed by acetone, embedded in Epon (Momentive Specialty Chemicals, Inc., Columbus, OH, USA), and dried in an oven at 60°C for 4 days.
For me, it's "The Baby-Sitters Club," Ann M. Martin's blockbuster middle-grade series.
The actor Jason Segel is venturing into children's books with a middle-grade series to be published by Random House, the publisher said on Tuesday.
Evolutionary colour changes dictated by shifting selection are suggested by many populations that show geographical or temporal clines (graded series of morphological characters).
In the first installment of "Allie Finkle's Rules for Girls," her new middle-grade series for Scholastic Press, Cabot has dialed back her tic of randomly capitalizing every fourth word (she's switched over to italics), the boy-craziness and the out-there premises.
In a recent phone interview, Ms. Clare spoke about the film, her forthcoming middle-grade series, writing with Holly Black (author of "Doll Bones" and "The Spiderwick Chronicles") and what readers can expect from "City of Heavenly Fire," the final novel in the "Mortal Instruments" series, due next May.
However, the vivacious Bella, drawn with affection and humor by Halpin (who also illustrates several middle-grade series), has her own idea of what's appropriate — candy for breakfast, no washing your hair ever, no such thing as bedtime — that doesn't jibe with that of her parents.
Dehydration of the specimens was performed by an increasing and graded series of ethanol (70 100 %), and then embedded in methacrylate-based resin.
Prefaced with "hunch" or "guess", this list of terms would reflect what many people consider a graded series from least to greatest degree of certainty.
Dehydrated root tip segments were embedded in Steedman's Wax (Polyethylene glycol 400 distearate : 1-hexadecanol = 9 : 1, w/w) as described by He et al. ([2004]) via a graded series Steedman's Wax : ethanol-1 : 1, 2 : 1, 1 : 0, 2 h each step.
