The treatment of aerobically growing E. coli with tellurite results in the the loss of the transmembrane proton gradient and depletion of intracellular ATP without affecting the pools of glycolytic intermediates [44], suggesting that the primary targets for tellurite are critical carriers involved in respiratory electron transport.
Similarly, Hilderbrand et al. [ 15] found that not only δN signatures of white spruce decline with distance from salmon-spawning streams, but that the activity of radio-collared brown bears were highly correlated with this same gradient of depletion.
Alternatively, decreasing (increasing) the deactivation or decay parameter value will show lower (sharper) gradients of depletion part of expression profiles (Response Rule 1, Additional file 1: Figure S1).
We tried two other techniques to eliminate debris: sucrose gradient and myelin depletion.
TLs were isolated from peripheral blood of healthy controls and RA patients, and from RA synovial fluid by Ficoll Hypaque gradient and magnetic depletion of non-T cells; purity was 99%.
This condition appears due to concentration gradients, with local depletion of nutrients and accumulation of waste materials [ 18].
Gradients of oxygen depletion were measured and respiration rates calculated as μmol molecular O2/10 cells/min assuming 100% O2=0.257 μmol O2/ml at 37 °C.
Edema formation in stroke causes an increase in cell volume, depolarization, breakdown of ionic gradients, cellular ATP depletion, and finally vasogenic edema in response to BBB breakdown [8].
Diffusion gradients or a depletion of Fe(II) was not observed in the anaerobic bulk soil immediately adjacent to metal flux maxima.
B lymphocytes were separated from peripheral blood using Ficoll-Paque PLUS (GE Healthcare) gradient centrifugation with a depletion of non-B cells (RosetteSep Human B Cell Enrichment Cocktail, RosetteSep Human CD3 Depletion Cocktail; Stemcell Technologies).
