Sentence examples for gradient centrifuge from inspiring English sources
Mononuclear cells were isolated by density gradient centrifuge using lymphocyte separation solution (Ficoll1.077 g/mL from TBD Biotech, Tianjing, China).
The cellular accumulation of [3H]-MTX, using a rapid gradient centrifuge technique for separation of melanoma cells from medium, was reduced in the presence of 70H-MTX.
The mice were sacrificed at 8 wks after transplantation; bone marrow MNCs were harvested by Ficoll density gradient centrifuge and stained with FITC-labeled anti-human CD45+ antibody and then analyzed by flow cytometry.
Blood samples were collected from all study subjects in heparin tubes and peripheral blood mononuclear cells (PBMC) were isolated using a Ficoll-Paque Plus density gradient centrifuge (GE Healthcare Bio-Sciences AB, Uppsala, Sweden).
For analysis of CNS-infiltrating cells, both spinal cord and brain were collected and minced from perfused mice, and mononuclear cells were isolated by gradient centrifuge with Percoll (GE Healthcare).
The resulting cell suspensions from these two techniques were transferred to a percoll gradient, centrifuged, and lymphocytes were recovered.
PMN-MDSCs have previously been found to be enriched among low-density granulocytes (LDGs) in density gradient centrifuged blood.
In this study we analyzed potential MDSCs in sepsis patients with different causative microorganisms, using total peripheral blood as compared to density gradient centrifuged blood.
MI fractions were purified on a 10%andd 28.5% sucrose step gradient centrifuged at 100,000 ×g for 2 h and collected at 10/28.5% interface.
Cell lysates were loaded on a 10 50% sucrose gradient, centrifuged at 150,000× g for 2.4 hr and absorbance at 254 nm was measured across the gradient (see Figure 3 figure supplement 3 for quantitation of polysome profile).
This digest was then layered on a 33%/77% Percoll density gradient, centrifuged for 30 min at 800 × g 4 °C and the non-parenchymal layer removed, pelleted and resuspended in 500 μl of PBS.
