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Allogeneic BMSCs were purified by density gradient centrifugation and centrifuged at 2500 rpm for 30 minutes.
Neutrophils were obtained by density gradient centrifugation.
Mononuclear cells were isolated from UCB by density gradient centrifugation.
Lymphocytes were separated with density gradient centrifugation.
PBMCs and plasma were isolated by whole blood gradient centrifugation on Lympholyte-Mammal (Cedarlane Labs).
48S mRNA-ribosome complexes were isolated by sucrose density gradient centrifugation.
The granules were isolated on Percoll density gradient centrifugation.
Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation using Histopaque 1077 (Sigma).
(b) Cell lysates from 3T3-L1 Ctrl) and 3T3-L1 LSD1 3T3-L1 LSD1atedifferentiatedere subjected to sucrose gradipocytestrifugation.
PBMCs from buffy coats were isolated using Histopaque-1077 (Sigma-Aldrich) density gradient centrifugation at 400 × g for 20 min.
Plasma membrane fractions were also obtained by homogenizing ~2 × 109 cultured cells followed by density gradient centrifugation.
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