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We examined all patients using a 3.0-T whole-body magnetic resonance system (General Electric Company, Milwaukee, WI, USA) with a 40 mT/m maximum gradient capability.
The primers were tested by conventional PCR on a Stratagene (La Jolla CA, USA) Robocycler with temperature gradient capability, using Ap strain HZ DNA as target.
MR imaging examinations of the whole nasopharynx were performed using a 3.0-T whole-body system (Signa Excite HD, GE Healthcare, Milwaukee, WI) with a 40-mT/m maximum gradient capability and a standard receive-only head and neck coil.
Structural MRI data were acquired using a 1.5-T Sonata MR imager (Siemens, Erlangen, Germany) with a standard quadrature head coil and maximum 40 mT m−1 gradient capability at the Oxford Centre for Clinical Magnetic Resonance Research.
HILIC was performed on an L-7100 pump system with quaternary gradient capability (Hitachi, Tokyo, Japan) using a TSK gel Amide-80 HILIC column (2.0 × 150 mm, 3 μm; Tosoh Biosciences, Tokyo, Japan) [25], [26], [27] with a flow rate of 200 μL/min.
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All NMR experiments were performed at 298 K on a Bruker Avance 800 MHz spectrometer with cryogenically cooled triple resonance probes equipped with pulsed field-gradient capabilities, and standard double- and triple-resonance through-bond correlation.
Two pairs of pumps were used for each mobile phase to create gradient elution capability.
A miniaturised medium pressure capillary LC (MPLC) system with gradient elution capability has been designed based on a flexible modular microfluidic system using primarily off-the-shelf low cost components to ensure wide accessibility to other analysts.
For example, a complete model of chemotaxis would require a description of the gradient sensing capability of cells together with a physical model for the cellular migration [ 3].
Pressurization of the packed capillary to prevent "air" bubble formation, gradient elution capabilities and thermostating devices allowing a greater flexibility in column designs have been implemented in CE instruments of several manufacturers.
The serosal system limits the full gradient-forming capability of the muscosal system.
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