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Of the areas tested, 45 were found to be grade four or worse (China grades water purity on a scale of one to five, with grade five being fit only for irrigation).
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HPLC-grade water and CH3CN were provided by Carlo Erba (Milan, Italy).
Millipore deionized grade water was used for all the experiments.
Molecular-grade water was used as a reaction negative control.
All dilutions were made with HPLC-grade water (J.T.Baker 4218).
MS-grade water and MS-grade acetonitrile was purchased from Carl Roth (Essen, Germany).
HPLC-grade water was mixed with HPLC-grade acetonitrile in the volume ratio of 1 1.
HPLC-grade water was prepared by double-distillation of deionized water.
HPLC grade water (minimum resistivity of 18.2 MΩ) was produced in-house by ELGA Millipore system (France).
For qPCR controls, the template was replaced by molecular grade water.
Negative control was carried out by using PCR grade water instead of cDNA template.
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