Sentence examples for grades of staining from inspiring English sources

For the statistical analysis, the six grades of staining were reduced to three grades: negative, [0/]; I, moderate ; and II, intense.

The four grades of staining intensities were scored as 0, 1, 2, and 3. Similarly, the five grades of staining patterns were scored as 0, 1, 2, 3, and 4. Thymidine phosphorylase up- or down-regulation was evaluated as the difference between the sample score after chemotherapy minus the sample score prior to chemotherapy for each patient.

The assessment of the grade of staining was determined using a blinded evaluation procedure by experienced pathologists.

The distribution was scored according to the number of positive cells; none (not stained), 0; focal (<1/3 of cells stained), 1; multi-focal (1/3 to 2/3 of cells stained), 2; and diffuse (>2/3 stained), 3; staining intensity was scored as: none (not stained), 0; lightly stained, 1; and strong staining, 2. The distribution and intensity scores were added to produce the grade of staining.

Immunohistochemistry using immunopurified anti-nm23 peptide antibodies was performed and the results of each case graded according to the degree of staining.

Staining was graded for intensity of staining according to previous description [ 31].

In contrast, 50 80% of lymphocytes present in the bronchial mucosae or infiltrating alveolar walls in inflammatory lungs showed a moderate nuclear staining and served as internal positive control with a staining intensity of 2 for grading the intensity of staining in neoplastic cells (from 1 to 3).

Although there is relative uniformity between the grading assigned by different investigators at the extremes of staining (grades 0 and ++++), variation is often most pronounced regarding the distinctions between the intermediate intensities of staining, such as between assigning a grade of + and ++ or between ++ and +++ for observed cellular density.

IHC staining was estimated by microscopy as the frequency (percentage) of stained cells as well as by the intensity of staining (graded from 0 to 3).

The assessment of the grade of staining was performed in a blinded way always by the same experienced investigators (IC, MIA) in high-power fields (×400) using standard light microscopy.

The intensity of staining (graded+ to +++) and the proportion of cells stained (scored in percentages) were evaluated.