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Exact(28)
They were fixed in neutral buffered formalin (10%%), and then dehydrated with grades of ethanol (70, 80, 90, 95 and 100%%).
The tissue sections were de-paraffinized in two changes of xylene, hydrated using descending grades of ethanol, and rinsed in a Tris buffer containing Tween 20 (DakoCytomation TRIS-Buffered Saline S1968/DakoCytomation Tween 20 S1966).
Sections were washed in distilled water and counterstained with hematoxylin for 1 min. And then sections were rinsed through ascending grades of ethanol with a final three changes of 100% reagent-grade ethanol, cleared in xylene and cover slipped with permount (Fisher Scientific).
Tissue sections were deparaffinized and rehydrated in descending grades of ethanol following standard protocols [ 34].
Tissues were then dehydrated through ascending grades of ethanol, infiltrated, and embedded in araldite CY 212.
Briefly, sections were dewaxed in xylene and rehydrated through different grades of ethanol up to distilled water.
Similar(32)
The fixed specimens were dehydrated in an ascending grade of ethanol, cleared in benzene, and embedded in paraffin.
Briefly, tissues were dehydrated in an ascending grade of ethanol, cleared and embedded in paraffin wax.
Coronal tissue sections were deparaffinized with xylene, rehydrated with descending grade of ethanol, and incubated with 3%H2O2/PBSS to quench the endogenous peroxidase activity.
Tissues were dehydrated in ascending grade of ethanol, infiltrated and embedded in low melting paraffin at 56°C in a heated oven.
Dehydration of the specimens was performed by an increasing and graded series of ethanol (70 100 %), and then embedded in methacrylate-based resin.
Related(20)
grades of fuel
concentrations of ethanol
grades of drinking
grades of ethyl
grades of liquor
gradations of ethanol
standards of ethanol
gradients of ethanol
degrees of ethanol
grades of gasoline
levels of ethanol
characteristics of ethanol
grades of petrol
grades of acid
grades of up
grades of instruction
grades of disbelief
grades of lactose
grades of ore
grades of secrecy
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