Sentence examples for graded solutions from inspiring English sources

The retinal samples were dehydrated in graded solutions of ethanol and passed through one change of propylene oxide.

Subsequently, cells were washed and stained with OsO4 and uranyl acetate, dehydrated in graded solutions of ethanol, and embedded in Epon resin.

Sections were stained with hematoxylin for 20 s using the HistoGene Staining Solution (Arcturus HistoGene, LCM Frozen Section Staining Kit, CA, USA) and dehydrated in graded solutions of ethanol for 30 s each.

After a survival period of 18 days, the animals were anesthetized, transcardially perfused with 4% paraformaldehyde and 0.2% glutaraldehyde, and the brains were removed from the skull, cryoprotected in graded solutions of sucrose (10 30%), frozen, and cut on a freezing microtome in the coronal plane at 40 or 50 µm, as described previously [27].

Slides were deparaffinised with xylene and rehydrated in graded solutions of ethanol and distilled water.

For immunohistochemical analysis, slides were deparaffinized and rehydrated in graded solutions of ethanol and distilled water.

After extensive washing in PBS, the cells were post-fixed in 1% w/v OsO4 for 30 min, dehydrated in a graded solution of ethanol and embedded in Epon.

Samples were dehydrated in graded ethanol solutions from 50 to 100% and in hexamethyldisilazane (HMDS, Ted Pella, Redding, CA, USA), then sputter-coated with platinum.

Slides were air-dried, dehydrated through graded ethanol solutions (70%, 95 % and 100), incubated in xylene and coverslipped using Eukit (Proscitech, Queensland, Australia) permanent mounting media.

The specimens were rinsed with distilled water and dehydrated in graded ethanol solutions with serial concentrations of 25%, 50 %, 75 %, 95 and 100%.

Slides were then counterstained in hematoxylin, dehydrated through graded ethanol solutions and cover slipped.