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Each mouse then completed two graded exercise tests separated by 48 hrs.
Moseley and Jeukendrup (2001) calculated the within subject CV on the basis of three graded exercise tests.
Laboratory personnel and the physician in charge of the graded exercise tests and randomisation procedure were blinded to the selection outcome of each participant.
Graded exercise tests (GXTs) with electrocardiographic monitoring were conducted at baseline in 5,783 individuals, providing the largest sample of systematic stress testing ever conducted in individuals with type 2 diabetes.
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Peak values were recorded for the maximal graded exercise test.
Respiratory gas exchange values were averaged every 30 s during the maximal graded exercise test.
During the first visit, they performed a maximal graded exercise test.
A graded exercise test to exhaustion (GXT) was then completed at a constant speed.
The maximum work rate was defined as the power output during the last stage of the maximal graded exercise test.
Main Outcome Measures: A graded exercise test with gas analysis was conducted on a bicycle ergometer for each subject.
Heart rate (HR) (RS800; Polar Electro, Kempele, Finland) was averaged every 30 s during both the maximal graded exercise test and the intermittent cycling exercise tests.
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