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The final cDNA reaction was diluted 1 20 in molecular biology grade water (Thermo Scientific).
The final lysate was diluted fivefold in molecular biology grade water.
After incubation, the beads were washed with IAP buffer and with mass spectrometry grade water.
Millipore deionized grade water was used for all the experiments.
Extracts were eluted in 50 µl of molecular grade water.
The negative specimen consisted of molecular biology grade water.
Similar(18)
Samples were diluted in IC-grade water (Sigma Aldrich).
The sample was vortexed, sonicated for 5 min and 150 μl MS-grade water was added.
Molecular-grade water was used as a reaction negative control.
All dilutions were made with HPLC-grade water (J.T.Baker 4218).
Injection-grade water and 0.9% NaCl were endotoxin-free in the limulus test (Charles River).
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