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Prior to extraction the peel was removed with a knife cleaned with HPLC grade solvent to avoid cross contamination.
The present study reports on the extraction of polyphenols especially flavanones from orange (Citrus sinensis L). peel by using ethanol as a food grade solvent.
Ultrasound assisted extraction (UAE) was applied for the extraction of polyphenol and antioxidants from pomegranate (Punica granatum L). peel using ethanol water mixture as a food grade solvent.
The HPLC conditions for the mobile phase were solvent A, ethyl acetate (HPLC grade); solvent B, acetonitrile/water (9 1, v/v); flow rate = 1 mL min-1.
For example, solution (5 mg/mL) of all samples will prepared in HPLC grade solvent mixture of acetonitrile/methanol/water (1 : 1 : 2; v/v/v) and filtered using membrane disc filter (0.45 μm).
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Reagent grade solvents were used.
HPLC grade solvents were used for the synthesis.
The HPLC grade solvents were purchased from VWR analytical, USA.
All the solutions were prepared using spectroscopic grade solvents.
Analytical grade solvents were purchased from Sigma-Aldrich.
All solvents were HPLC grade solvents, filtered through a 0.45-μM membrane (Millipore, U.S.A).
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