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300 ng of RNA was used as a template for reverse transcription (30 min at 50°C), followed by activation of the HotStarTaq polymerase (15 min at 95°C), 10 cycles of PCR (30 sec at 95°C, 30 sec at 68°C decreasing the temperature by one grade Celsius each cycle until reaching 58°C, 30 sec at 72°C), 30 cycles of PCR (30 sec at 94°C, 30 sec at 58°C, 30 sec at 72°C) and final 7 min extension at 72°C.
GCS denotes Glasgow Coma Scale, DPB diastolic blood pressure, HR hear rate, T bodily temperature (grade Celsius), CSF cerebrospinal fluid.
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In June 2010, he developed pancytopenia with low grade fever 37-388 degree Celsius).
Degree Celsius.
The LST results are all in degree celsius.
Those plates were incubated at 37 degree Celsius overnight.
The cycling conditions were initial denaturation at 95 degree celsius for 2 min followed by 40 cycles with a profile of 95 degree celsius for 1 min, 59 degree celsius for 1 min, 72 degree celsius for 1 min, and a final extension at 72 degree celsius for 5 min.
The 1 1 ratio for each degree Celsius and Kelvin still applies while converting Kelvin to Celsius.
The industrial furnace next to the space was once used to destroy toxic materials at temperatures of 900 degrees celsius, but these military grade incinerators will now be set up to burn any surplus cannabis – another requirement from the state.
Symptom intensity of redness, swelling and fever was graded by millimeter of reaction and degrees Celsius of fever, respectively, and all other symptoms were graded by the subject using a pre-defined scale.
Use a 200 degree Fahrenheit (93 degrees Celsius) oven.
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