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We found that two primer pairs, flanking −1421 to −1333 and −1191 to −1118 respectively, exhibited significant enrichment in GR ChIP (Fig. 1B), but not with IgG alone (data not shown).
(E ) GR ChIP in DMI-treated hMSCs comparing LD to HD cells at 7 genomic sites and a non-specific control (INS).
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DJS and MAL designed and carried out CEBPB and GR ChIP-seq experiments.
GR ChIP-seq peaks are placed into three groups, pre-programmed, re-programmed, and un-programmed.
Genome distribution of GR ChIP-Seq peaks was analyzed using the CEAS package [ 49].
GR ChIP-Seq was performed on fixed and sonicated chromatin, whereas histone modification ChIP-Seq was performed on native chromatin fragmented by MNase digestion.
When we examined GR ChIP-seq peaks, GR motifs with a nearby AP-1 motif are better bound, with a clear inflection at 150-bps.
The data of c-Jun ChIP-seq peaks and GR ChIP-seq peaks upon A-FOS is from the study [ 22] deposited at NCBI with SRA number of SRP007111.
GREAT analysis using all GR ChIP-Seq peaks (a), as well as subsets of GR peaks with central remodeling (b), minimal remodeling (c), non-central remodeling (d), or phased remodeling (e).
To examine the cell type specificity of this localized nucleosome depletion, we used publicly available GR ChIP-Seq data for the A549 lung carcinoma cell line from the ENCODE project [ 24– 26] (filename at UCSC Genome Browser: wgEncodeAwgTfbsHaibA549GrPcr1xDex50nmUniPk.narrowPeak.gz).gz
Co-occurrences of AP-1 motif with 1-bp variants of GR motifs are always higher than those with the canonical GR motifs in the GR ChIP-seq peaks, although their binding intensity is lower than the canonical GR motifs (Table 2, Additional file 1: Figure S7B).
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