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Utilization was based on receiving one or more paid pharmacy claims where the GPI was for an opioid or non-steroidal anti-inflammatory drug (NSAID) therapy (GPI code 661000xx).
Utilization was based on receiving one or more paid pharmacy claims where the MediSpan© Generic Product Identifier (GPI) code was for a bisphosphonate (GPI-6 30.04.20), calcitonin (GPI-6 30.40.30), selective estrogen modulator (SERM) (GPI-8 30.05.30.60), or estrogen replacement therapy (GPI-4 24.99, 55.35).
To make VAC-gB, the gB-GPI coding sequence was amplified from pBRAD-gB [27], again with 5' AvrII-restricted and 3' HinDIII-restricted primers, and cloned into pMJ601 as for VAC-gHL.
To make VAC-gHL, the gHL-GPI coding sequence was amplified from pBRAD-gHL using 5' AvrII-restricted and 3' HinDIII-restricted primers and cloned into the NheI/HinDIII sites of pMJ601 [43].
These genes are directly related to cell wall compensatory responses: the CWI pathway MAP kinase SLT2 and its homolog MLP1 (YKL161c); the known CWI pathway downstream targets PRM5; the PIR-CWP PIR3; and four genes coding for GPI-anchored proteins (YLR194c, YPS3, SVS1 and YJL171c).
In this system, yeast is transformed by introducing fusion genes coding cellulolytic enzymes and the anchoring domain of GPI protein.
Bm86 (R. microplus), Ba86 (R. annulatus) and Bd86 (R. decoloratus) coding regions (nucleotides 58 1884 of the coding region of Bm86 reference sequence; GenBank accession number M29321) lacking the signal peptide and GPI anchor sequences were amplified by RT-PCR.
To make gHL-GPI, the gL coding sequence lacking its stop codon and the first 13 amino acid residues of its signal peptide [22] was amplified by PCR (Phusion DNA polymerase, New England Biolabs, Hitchin, U.K). with NotI-restricted primers and cloned into the NotI site between gH and its GPI anchor in pBRAD-gH [22] to make pBRAD-gHL.
29 Chemotherapy agents were identified using generic product identifier (GPI) and Healthcare Common Procedure Coding System (HCPCS) codes.
Hence, a TM system must be designed compatible with good programming incentives (GPI), i.e., writing efficient code for the overall system should coincide with writing code that optimizes an individual thread's performance.
The GPI transamidase recognizes a C-terminal GPI-anchor signal peptide (GPI-SP), which is cleaved concomitantly to the attachment of the GPI-anchor.
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