Exact(26)
The analysis of the archaeal 16S rRNA gene library, allowed the definition of 12 OTUs out of 53 good quality sequences.
The obtained sequences were compared to the database of the Ribosomal Database Project (RDP) (Cole et al. 2013) using the SeqMatch analysis tool to identify the closest relative sequences (uncultured bacteria and isolates, only good quality sequences >1200 bases).
DOTUR analyses of the 16S rDNA bacterial sequences from P soil sample allowed the definition of 105 OTUs from 145 good quality sequences, generating 83 singletons and 14 doubletons.
A total of 68 good quality sequences derived from the catabolic gene libraries from P Soil were analyzed and seven distinct protein groups were obtained based on the distance level previously defined for OPFs, which were later confirmed by Blast score ratio analysis performed in BlastP database.
The amplified material present in strong bands obtained with PCR was sufficient for good quality sequences in direct automated fluorescent sequencing.
Experimental sequence data were analysed and assembled with CodonCode Aligner (http://www.codoncode.com/aligner) and the contig containing good quality sequences was compared with the sequences available at the non-redundant database at NCBI using BLASTX.
Similar(34)
Only where we could attain good quality sequence data for variable domains II and IV of ompA did we accept the identification data as accurate.
The shortest accepted length of good quality sequence was 18 bp.
Overall, the PGM and MiSeq approaches generated good quality sequence data rapidly with relatively high coverage.
One disadvantage of overgos is that good quality sequence information must be available from which to design the probe.
Randomly selected 179 SFPs were used for validation by Sanger sequencing and good quality sequence data were obtained for 99 genes of which 75 genes showed sequence polymorphisms.
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