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However, the ammonium is good nitrogen source for the phytoplankton growth, the unfavorable concentration causes growth inhibition due to toxicity and/or acidifying the culture media during algal growth (Garcia et al. 2006; Xin et al. 2010).
Among these, casein proved to be a good nitrogen source for stimulating protease production (156.80 U/mL).
Consistent with its status as a good nitrogen source for growth, glutamate is readily converted into ammonia and glutamine to provide the nitrogen required to carry out protein synthesis and other cellular processes.
Similar(57)
When a good nitrogen source is present, NCR shuts down the pathways for the use of poor nitrogen sources.
By binding to transcription factor Gln3, Ure2 suppresses expression of genes required for uptake of poor nitrogen sources when a good nitrogen source is present.
Present at the plasma membrane when the nitrogen source is poor, it undergoes ubiquitin-dependent endocytosis and degradation upon addition of a good nitrogen source, e.g., ammonium.
This direct vacuolar sorting is normally observed for native Gap1 when the Npr1 kinase is inactive or when a good nitrogen source is available, causing Npr1 inactivation via the TOR signaling pathway [8], [25].
When a good nitrogen source is added to the medium, Npr1 is proposed to be inactivated via the TOR signaling pathway [26].
nit-6 encodes the structural gene for nitrite reductase and is only expressed when N. crassa is cultured on medium containing nitrate and lacking a good nitrogen source (typically glutamine or ammonium) (Exley et al. 1993).
In the absence of a good nitrogen source, GLN3 is activated and in turn activates the transcription of NCR-sensitive genes.
Hence, down-regulation from the cell surface of Gap1-124 seems to rely on mechanisms similar to those induced by good nitrogen sources.
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