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Coomassie stain and Western blot analysis of the SDS-PAGE gel revealed similar good level of expression of all APE1 proteins except K98A/R185A double mutant (Supplementary Fig. S3).
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However, some of the higher molecular weight proteins were expressed, in fact, proteins with has many as twelve TMH were observed to have good levels of expression.
It is known from transgenic mouse studies, which utilize the mPlp1 promoter to drive expression of the lacZ reporter, that inclusion of mPlp1 intron 1 is essential for attaining good levels of expression in brain, concurrent with the active myelination period (Li et al., 2002).
While the natural full-length gene sequences for most of these Y. pestis proteins did not display a good level of protein expression in vitro when delivered by a DNA vaccine vector, the overall immunogenicity of these wild type gene DNA vaccines was low in eliciting antigen-specific antibody responses and gene sequence modifications improved both of these parameters.
All the others strains possessing an N-terminal fusion partner yielded a good level of protein expression.
These properties of Clone A1 were stable during 60 days of growth under conditions of Dox repression, with tight promoter regulation in the presence of Dox and a good level of inducible expression of GFP upon Dox withdrawal being maintained throughout.
It was sufficient to relieve translation inhibition and to obtain good levels of protein expression.
Additionally, in order to achieve good levels of pac expression, we cloned the IRES of the encephalomyocarditis virus between the EGFP and pac genes which allowed the bicistronic mRNA transcript to be translated from the 5' end and from the IRES sequence, so that the pac gene was also under the control of the strong synthetic late promoter.
Power law expressions provide a good level of accuracy for curve fitting.
A good level of fitness is essential.
One of the lines, TLG 498, showed good levels of EGFP-F expression, in more than 90% of pyramidal neurons in the neocortex and hippocampus.
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