The yield and purity of the biotinylated aRNA samples were determined in a BioPhotometer (Eppendorf) and only samples of good integrity were further processed.
Only samples of good integrity were further used and 15 μg of the fragmented samples were hybridized to GeneChip® Rat Genome 230 2.0 Arrays (RAT230_2 chip, Affymetrix) and subsequently scanned.
Large RNA integrity was assessed using the RNA 6000 Nano LabChip Series II Assay with the 2100 Bioanalyzer System (Agilent Technologies, Palo Alto, CA, USA) which generated the RNA integrity number (RIN) to ensure that only RNA of good integrity was used in these experiments (RIN range 7.0 - 9.5).
Electropherograms and gel-like images were evaluated using the Agilent 2100 Expert software (version B.02.03; Agilent Technologies, Palo Alto, CA, USA), which generated the RNA integrity number to ensure that only RNA of good integrity was used in these experiments (RNA integrity number range, 7.6 to 9.5).
KTP and its isomorphs pole anisotropically, favouring domain growth along [010] on the conventional setting of the cell with a = 12.819 (3), b = 6.399 (1), c = 10.584 (2) Å (Thomas et al., 1990 ▶). Gratings of good integrity were produced with walls designed to be parallel to (100) planes (a-poled), whereas the b-poled gratings with walls parallel to (010) were of a poor quality.
Good gate oxide integrity is further evidenced by its frequency-dependent C-V characteristics.
Citing Bryan Ferry as an exemplar of good taste and artistic integrity is so ludicrous as to beggar proper rebuttal.
If this happens it will surely be premature: Giles, while a good man of real integrity, is part of the general malaise as evidenced by the performances of both short-form teams in Australia these past few weeks.
"Anything that shows the industry has integrity is good for its long-term growth," she said.
