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The chitosan-collagen/PLLA uncoated scaffolds and alginate gel coated chitosan-collagen/PLLA scaffolds showed good cell proliferation.
Cell culture using gelatine 2.0% (SaG2Fu) and 4.0% (SaG4Fu), showed good cell proliferation; more than 60 80% that with Sa alone.
Furthermore, the PEI coating shows an anti-inflammatory response, and good cell proliferation and migration were demonstrated in vitro using osteoblast SAOS-2 cell line.
From analysis of the cell viability data, it is evident that FGM supports good cell proliferation after 2, 3, 4 days culture.
Above results suggest that the collagen type I-modified PCL/gelatin scaffold was successful in maintaining characteristic shape of fibroblasts, besides good cell proliferation.
The MPT sample was not only better in supporting osteoblast cell viability and good cell proliferation, but also was beneficial to immobilize the silver loaded microspheres to achieve a stronger antibacterial effect than PT.
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In vitro cell culture studies by seeding osteoblast cells over the composite scaffold showed good cell viability, proliferation rate, adhesion and maintenance of osteoblastic phenotype as indicated by MTT assay, ESEM of cell scaffold construct, histological staining and gene expression studies, respectively.
Cell matrix interaction was also done which inferred good cell adhesion, proliferation, and secretion of ECM on matrices.
The air spinning was designed to process a scaffold that would support good endothelial cell proliferation.
In vitro tests showed good MG63 cell proliferation, as confirmed by the results of the MTT assay and scanning electron microscopy (SEM) observations, with a higher cell viability on EC-2 foam 7 days post-seeding.
The most appropriate culture conditions for good T cell proliferation included stimulation with anti-CD3 and anti-CD28 coated microspheres, and propagation in Aim V serum-free media with 20 U/ml interleukin-2 (IL-2), supplemented with decreasing concentrations of serum for the initial 8 days.
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