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Up to nine goldfish were used per episode, so they could be replaced when necessary.
Furthermore, IHNV, VHSV, IPNV, KHV, NDV, AIV (H5, H7, H9), CO cell, EPC cell, FHM cell, eggs of sturgeon and salmon, tissues (muscle, pancreas, gill, spleen, fin, brain, kidney) of ornamental carps, common carps, goldfishes, and grass goldfish were used as control templates to verify the specificity of the method.
Adult male and female goldfish were used to optimize in vivo transfer methods and to determine if water-borne estrogenic chemicals activate ERE-TK-LUC injected into the adult brain.
Although 77 goldfish were used in the experiment, data from 56 fish were not subjected to further analysis due to insufficient recording quality, loss of the unit before the completion of conditioning procedure or ambiguities in the identity of recorded neurons.
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Two species of fish, common carp and goldfish (Carassius auratus), were used separately in three fertilizer treatments, namely with 0-0-0, 0-8-8-2aN-P2O5-K2Oounds of N-P2O5-K2O per acre1 per application.
Experimental microarray data from the AURATUS goldfish environmental genomics project were used to define seasonal gene expression information (Table S1).
Briefly, three groups of six weight-matched goldfish (BW = 33.71±9.71 g) were used for central injections of 25, 0.5 and 0 ng/g BW gfnesfatin-1.
He delivered a speech on goldfish in New Education Review in 1926, in which he suggested that goldfish could be used in biology courses to increase students' interest in biology, and to demonstrate the morphology, physiology, embryo and development, variation and inheritance of animals as experimental materials (Chen, 1926).
Goldfish have been used for cold acclimation studies, which have focused on changes in glycolytic and oxidative enzymes or alterations in lipid composition in skeletal muscle.
Our results showed that in vivo transfection in the goldfish telencephalon can be used to study the regulation of ERE-driven expression by an estrogenic pollutant.
Icing patterns need to be laid out like a stream of goldfish turd rather than dragged as if you were using a pen.
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