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The goldfish brain has a remarkable capacity to produce E2 from testosterone because of very high aromatase activity (Callard et al. 2001; Pasmanik and Callard 1988).
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His brain had compartments.
In the present study, by analyzing multiple female goldfish brain microarray datasets, we have characterized global gene expression patterns for a seasonal cycle.
To date, many other well established neuropeptides with mammalian orthologues have been characterized within the NLT of the goldfish brain.
As expected, the antibody targeted full-length NUCB2 in rat and goldfish brain samples (Figure 5C).
Total RNA was extracted from goldfish brain using the TRIzol® RNA isolation reagent (Invitrogen, Canada).
Anatomical localization and nomenclature were based on the goldfish brain atlas [26].
A sagittal view of the goldfish brain stained with nesfatin-1 antiserum, revealed a large number of ir cell bodies within the goldfish hypothalamus (Figure 6A F).
Following bioinformatics analysis, we collected goldfish brain samples at six seasonal time points for experimental verification of gene expression patterns.
On the basis of this meta-type dataset, we identified four clear seasonal gene expression patterns in female goldfish brain.
No band representing the processed nesfatin-1 (expected size: ∼9.5 kDa) was detected in either rat or goldfish brain samples.
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