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A total of 158 [A/JxAKR/J]F2 mice were genotyped at the McGill University and Genome Quebec Innovation Centre (Montreal, QC, Canada) using Sequenome iPlex Gold technology and a custom panel that contained 225 informative SNPs distributed across the genome.
Genotyping was performed using iPLEX Gold technology [ 36] and Mass ARRAY high-throughput DNA analysis mass spectrometry (Sequenom, Inc).
SNPs were genotyped using MALDI-TOF spectrophotometric mass determination of allele-specific primer extension products with Sequenom MassARRAY platform Sequenom, Inc., San Diego, CA, USA and iPLEX Gold technology (Sequenom, Inc.,).
A total of 90 SNPs were genotyped, 85 using MassARRAY iPLEX Gold technology (Sequenom, San Diego, CA, USA), and five using Taqman 5' nuclease assay (Applied Biosystems, Foster City, CA, USA) in 719 cases and 748 controls.
For this, 57 variants across these nine genes were genotyped in HAPE patients (n=225), HAPE controls (n=210), and highlanders (n=259) by Sequenom MS (TOF -based MassARRAY® plaTOF -basedg iPLEX™ Gold technology.
These polymorphisms were genotyped by the Sequenom MS (TOF -based MassARRAY® plaTOF -basedg iPLEX™ Gold technology in the three study groups, coMassARRAY®25 HAplatform0 HAPE-c, and 259 HLs.
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Genotyping was carried out by Sequenom iPLEX Gold MassARRAY technology at the McGill University and Génome Québec Innovation Centre, Montréal, Canada.
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