Sentence examples for gold labelled from inspiring English sources
In this study we demonstrate gold labelled ubiquitin predominantly on the intracellular side of the FDC plasma membrane, consistent with FDC ubiquitin production.
The cells were incubated with a ZnT-3 antibody (1∶2000) (kindly provided by Professor R. Palmiter, Seattle, USA) at 4°C overnight, rinsed in TBS and 1% (v/v) triton, and incubated with 10 nm colloidal gold labelled secondary goat anti-rabbit antibody, 1∶50 dilution, (Aurion) for 1 hour at room temperature.
Moreover, the recognition of these fibrils by gold labelled specific primary antibodies confirmed their correspondence with PrPsc.
A volume of 60 μL of unprocessed urine is added to the sample pad where colloidal gold labelled antibodies bind to LAM present in the sample.
The position of the gold labelled probes was verified on subsequent CT scans (except in Patient 5 were the catheter was inserted very late and Patient 9 were the catheter was removed very early).
Since the gold labeling influences the alignment procedure, single particle averaging was not performed on gold labeled particles instead, about a hundred images were visually analyzed (Figure 1C, see also Figure S2).
In order to quantify the labelling density and its variations in the different nuclear domains, the primary antibody was immuno-gold labelled and the sections were observed by electron microscopy to determine the number of electron dense particles per surface area (Figure 3D F).
Colloidal nano-gold labelled SPA was used as a detecting reagent which could bind specifically to the Fc portion of immunoglobulin from many mammals.
When immuno-gold labelled and used for EM, MW8 detected the fibrillar but, surprisingly, not oligomeric Htt that had been extracted from R6/2 brains.
However, histologic studies using colloidal-gold labeled ILs demonstrated efficient intracellular delivery in tumor cells, while non-targeted liposomes accumulated within stroma, either extracellularly or within macrophages.
Fluoro-Gold labeled neurons were detected by direct fluorescence.
