The tuned-for-gold detectors range in price from $500 to $5,000 and are available from online specialty shops like Kellyco Metal Detector Superstore (www.kellycodetectors.com).
The eluate was monitored at 280 and 254 nm using a Gold system detector (Beckman, USA).
Data were collected using a mosaic Gold CCD detector positioned 3.7 m from the 1 mm diameter capillary containing the samples, using 18 keV incident radiation.
Teflon capillaries at 70 °C connecting the recoil chamber, where the ununtrium atoms were synthesized, and the gold-covered detectors: the ununtrium atoms would be carried along the capillaries by a carrier gas.
While about ten to twenty atoms of 284Uut were produced, none of these atoms were registerd by the gold-covered detectors, suggesting either that ununtrium was similar in volatility to the noble gases or, more plausibly, that pure ununtrium was not very volatile and thus could not efficiently pass through the Teflon capillaries at 70 °C.
Among them, C9 had highest affinity, best dose response behavior, lowest limit of detection, and highest specificity and was chosen to be labeled with colloidal gold as the detector reagent and applied on the conjugate pad.
Two MAbs specific to isolates of E. tarda were obtained, and one of them (25C1) was selected to conjugate with colloidal gold as the detector antibody.
Monosaccharides were detected using a gold pulsed amperometric detector set on waveform A, according to the manufacturer's instructions.
FAD in column eluate was detected using a Beckman System Gold 168 photodiode array detector set at 229 nm.
The Dionex chromatography is equipped with a CarboPac PA1 (Dionex P/N 035391) 4 mm × 250 mm ion-exchange column and a Dionex ED 40 pulsed amperometric detector with gold working electrode and solid state reference electrode.
