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The grids were subsequently washed by placing them, successively, on 10 drops of incubation buffer (5 minutes on each drop), after which the sections were incubated with the gold-conjugated secondary antibody by letting them float on drops containing the gold conjugate reagent (diluted 1∶20 in incubation buffer) for 60 minutes at room temperature.
The gold conjugate shows double simultaneous stimulation-induced drug release behavior in the presence of both esterase and high concentrations of glutathione.
The TEM image also shows a much larger number of microspheres phagocytosed in relation to the numbers of gold conjugate.
6 nm goat anti-mouse IgG gold conjugate and 10 nm goat anti-rabbit IgG gold conjugate (Aurion) were from Electron Microscopy Sciences.
Negative controls were incubated with the gold conjugate alone and then counterstained with uranyl acetate and lead citrate.
The anti-TC was used at a dilution of 1∶25 and the secondary antibody at 1∶50 (Gold conjugate, anti-rabbit IgG, Ted Pella Inc., USA).
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A 1000-fold enhancement of the sensor's sensitivity is achieved when using gold conjugated monoclonal antibodies instead of free antibodies.
incubation at room temperature, samples were washed with TE buffer (pH 8.0) and probed with colloidal gold conjugated anti-rabbit (5 nm) and anti-mouse antibodies (10 nm) (SPI supplies).
Using colloidal gold conjugates on immunodecorated cells, we used electron microscopy to show that AMI6 mAb targets an exposed antigen at the surface of A. minutum.
The Colloidal gold conjugates were printed on conjugate pad at optimized concentration described later and dried at room temperature.
For usual 15-nm colloidal gold conjugates, the minimal detectable amount of hIgG is about 4 ng.
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