Exact(4)
The Au peaks are related to gold coating for the sample preparation before taking SEM images and it is not an impurity in the composite.
Additional attempts to optically inject IP3-loaded liposomes without the gold coating for 120 s consistently showed no observable Ca2+ response.
The dried samples then allowed to gold coating for 60 s before the images were captured using FEI Quanta 200 SEM using environmental detector.
The samples were mounted on aluminium cylinders with silver paste and placed in a high vacuum evaporator for gold coating for 6 min in a metal ionizing JEOL JFC-1100 (Fine Coat® ion Sputter, JEOL Ltd ,Tokyo, JP).
Similar(56)
The samples were mounted on aluminum stubs using double-sided carbon-coated tape and were vacuumed and gold coated for 10 min prior to analysis by using sputter coater.
Specimens were gold coated for 30 s at 35 mA.
The samples were gold coated for 60 s using a 10-mA current.
The sample powders were deposited on a carbon tape before mounting on a sample holder and gold coated for EDAX.
AFM images also confirmed XPS measurements, especially for samples treated by plasma for 60 s and gold coated for 300 s, on which irregular large clusters covered a relatively large portion of the PEEK surface; therefore, the gold concentration was slightly increased.
The dried samples were gold coated for better imaging using a sputter coater (Vacuum Tech, Bangalore, India).
Samples were then air-dried at room temperature, mounted on a stub, and placed in vacuum chamber of SEM gold coating apparatus for gold coating (2.5 kV, 20 25 mA for 120 s).
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