There are other methods that use GO vocabulary for protein function prediction methods including GOblet, GOfigure and GOtcha.
Segments underwent two mean inclusions: (1) in paraffin to obtain semi-seriated sections of 7 μm thickness each and subjected to hematoxilin and eosin (HE) staining and (2) in historesin (LKB, Technovit 7100) to obtain semi-seriated sections of 7 μm thickness each to histochemically identify glycoproteins present in intestinal goblet cells using the PAS method.
Many methods, such as GOblet, GOFigure, and Gotcha, are designed based on the BLAST search.
To quantify goblet cell hyperplasia, we used the method described by Padrid et al 20 The pathological changes were evaluated according to the modified five-point scoring system (grade 0 4) and are expressed as the percentage of goblet cells in the epithelium scored as follows: grade 0 (no goblet cells); grade 1,<25%; grade 2, 25%50%%; grade 3, 51%–75%; grade 4, > 75%.
Images of sections obtained using the PAS histochemical method were used to quantify goblet cells present in 50 images per animal (40× objective).
Now, Mitrovic et al. have used genetic methods to study 7343 gene products in goblet cells derived from a human colon.
Goblet cells were identified by a combined AB-PAS staining method.
The Alcian blue/periodic acid-Schiff staining method and hematoxylin-eosin staining were used to detect goblet and Paneth cells, respectively.
A further small and curated dataset of 28 targets was evaluated and compared with the following function prediction methods available on the web: JAFA [23], Blast2GO [22], GOtcha [20], PhyDBAC [24], GOblet [25], PFP [26], InterProScan [27].
Upstairs in the galleries, a goblet repaired long ago with a wooden foot provides an example of archaic glass restoration methods.
