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This can be seen especially at the GO map 2 and 3 relationship-level categories (Table 1 and supplemental material) using the multi-species GO browser (DrZOOview2.0 ).
The Gene Ontology (GO) categories of genes that were differentially expressed in CLL patients and normal controls were analysed with the 'PANTHER Classification System' GO browser tool.
Gene Ontology analysis using NetAffx GO Browser, and DAVID Functional Classification Tool [54] were used to identify the main functional groups over-represented among the differentially expressed genes.
Gene Ontology (GO) analysis using NetAffx GO Browser identified that 228 (57.3%) out of 398 genes differentially expressed between the strains were involved in metabolism, with a number of biological processes identified.
Identification and enrichment of GO terms within significantly differentially regulated sets of genes were obtained using the GO Browser function in GeneSpring GX with a Benjamini-Hochberg corrected p-value <0.05.
For DAVID and NetAffx GO Browser we have uploaded a gene list, in form of Affymetrix GeneIDs, of 399 differentially expressed genes, Biorag actually required GeneBank accession numbers and log ratio changes for the 3 groups.
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QuickGO is updated weekly with GO annotations and nightly with GO term information making it one of the most up-to-date GO browsers available, this is a critical feature of GO browsers, and GO analysis tools in general, due to the constant growth and updating of both the ontology and annotations.
In addition to their inclusion in dedicated GO browsers, GO annotations are also imported into many of the top biological databases, including UniProtKB, Ensembl, EntrezGene and GeneCards.
GO browsers, such as AmiGO (http://amigo.geneontology.org/) and QuickGO (http://www.ebi.ac.uk/ego/), provide information retrieval capabilities, allowing for manual comparisons of genes and their annotations.
Unfortunately, there are some GO browsers where there is a long lag between updates [e.g. Gofetcher (http://mcbc.usm.edu/gofetcher/home.php) (5), GenNav (http://mor.nlm.nih.gov/perl/gennav.pl)] requiring users, sometimes unwittingly, to use old data.
Currently there are 48 tools for gene expression and microarray analysis and 20 GO browsers, all of which are listed on the GOC tools web page (www.geneontology.org/GO.tools.shtml).shtml
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