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Each serum sample was separated by 10% SDS-Polyacrylamid-gelelectrophoresis, transferred to PVDF membrane and detected with a primary antibody against PCG1-α (polyclonal IgG antibody coupled to HRP (Antibodies-online, GmbH) produced in goat, dilution 1 500 in TBST (is mixture of Tris-Buffered Saline and Tween 20) supplemented with 5% milk powder, incubation over night at 4°C).
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hTR, containing a single 5′-thiophosphate functionality, was generated by in vitro transcription prior to reaction with the maleimide derivative of the fluorophore ATTO 647N (ATTO-TEC GmbH) to produce 647N−hTR, which we have previously demonstrated does not impair telomerase catalytic activity (21).
Crelux (CRELUX GmbH, Martinsried, Germany) produced co-crystals of a human truncated mutant casein kinase 1 δ (CK1δ1−316, R13N) with compound 5 that diffract to 1.7 Å resolutions at the ESRF synchrotron radiation source and determined the X-ray structure.
R.H. is co-founder of Neaspec GmbH, a company producing scattering-type scanning near-field optical microscope systems such as the ones used in this study.
A passive femo-femoral shunt flow generated by the arterial blood pressure through a lung assist device (iLA NovaLung® GmbH, Talheim, Germany) produces sufficient CO2 extraction and a moderate improvement in arterial oxygenation at a flow rate of approximately 1.0 1.5 l min−1.
The ozone treatments were performed with a Fischer Technology Ozone Generator 500 (i-Fischer Engineering GmbH, Waldbüttelbrunn, Germany) producing a continuous flow of ozone.
The 532-nm beam was directed to pump an optic parametric oscillator (Levante Emerald, APE GmbH, Germany) to produce a 6-ps tunable wavelength pulse train as the pump beam, and it was tuned to 909.2 nm for a lignin resonance frequency of 1600 cm−1.
The catalyst, Pd on porous granules, was installed in the reactor in KATAPAK elements, produced by Sulzer GmbH.
In 2010, German company Milchkristalle GmbH patented "nocturnal milk" and produced a powder from milk collected between the hours of two and 4am.
The Pd/C air electrode showed improved catalytic activity at high current densities (≥ 50 mA cm−2) and enhanced durability compared with two commercial Pt/C air electrodes produced by Gaskatel GmbH and Johnson Matthey.
DVB-ST polymeric particles (dp = 75 μm) were produced by Polymerics GmbH (Berlin, Germany); their characteristics are listed in Table 1.
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