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The GM was placed in the middle of the box, and the gas phase and vacuum phase were divided by the fixed GM.
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Hydrogel encapsulating various concentrations of GM-CSF was placed in the lower compartment of the transwell, while 0.5 million bone marrow-derived dendritic cells (BMDCs) or bone marrow-derived macrophages (BMDMs) were seeded in the upper compartment.
Accurately weighed 5 gm of NANE was placed in reciprocating shaker (Remi Corp., Mumbai) at 60 cycles/min for 24 h at room temperature.
Ammonium carbonate (215 gm, 4 mols) was placed in a 1 litre three-necked round bottom flask, which was fitted with a thermometer, a dropping funnel, and a bent tube attached for distillation to a short condenser.
It was placed next to non-GM tomato purée.
Briefly, hydrogels encapsulating various amounts of GM-CSF were placed in the lower compartment of the transwell, while the bone marrow-derived dendritic cells (BMDCs) and bone marrow-derived macrophages (BMDMs) were seeded in the upper compartment.
The GM-HFIT team commented that more focus could be placed on education sessions specifically for PNs, given that they often see patients for annual reviews.
Non-adherent cells were placed in fresh growth media (complete media containing 1000 U/mL of GM-CSF and IL-4) to generate DC.
The value of gm was estimated by the AN Ci curve-fitting method82.
The function gm is defined as (B.5 where (B.5 where
where gm is the channel transconductance of FET gm = ∂ Ids / ∂ Vgs.
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