First, the one-to-two mapping of Pv to Gm sequences provides further compelling evidence that a major duplication event is part of the history of the soybean genome [ 25].
Although these strains cluster together according to recA, tly, camp5, and gms sequences, they are distinct according to fba, coa, and zno sequences (Figure S1).
Among the limitations, it should be pointed out that since this approach is based on the detection of non-GM sequences in a bulk sample it can not account for the hemi- or homozygous state of individual seeds.
It can be observed that: GM-specific methods (i.e. event-, construct-, element-specific) do not detect any sequence in the ENA plants contigs dataset (emblconexp bars in Figure 1 A), where GM-sequences are not expected to be present.
GM-specific methods (i.e. event-, construct-, element-specific) do not detect any sequence in the ENA plants contigs dataset (emblconexp bars in Figure 1A), where GM-sequences are not expected to be present.
These residues are part of a conserved motif R292XLGXGD298 (the GM-linker motif using Pfu sequence numbering) present in all SRP54 sequences and located between the helix α7 at the C-terminal part of the G domain and the linker.
The corresponding EST clones for auxin down regulated ADR 6, ADR 11 and ADR 12-2 [Clone ID: Gm-r1021-3058, Gm-c1021-2201 and Gm-r1088-6964 respectively] were purified, sequenced and used as radio-labeled probes in an RNA blot that contained total RNA from the imbibed seed (stage1, reference tissue) and the remaining six different stages (2 7) from one of the biological replicates previously used.
Variants of this full-length form (383 amino acid residues) were generated by shifting the BamHI-restricted PCR primer further 5' in the gM coding sequence.
The gM coding sequence without its stop codon (genomic co-ordinates 56950 55796, 383 amino acids) [9] was cloned as a BamHI/XhoI-restricted PCR product into the BamHI/XhoI sites of pEGFP-N3 (Clontech, Palo Alto, CA).
However, all of the subclasses of mRNA include GM-CSF sequences at the 3′ end of their mRNA.
