First, the one-to-two mapping of Pv to Gm sequences provides further compelling evidence that a major duplication event is part of the history of the soybean genome [ 25].
Although these strains cluster together according to recA, tly, camp5, and gms sequences, they are distinct according to fba, coa, and zno sequences (Figure S1).
It can be observed that: GM-specific methods (i.e. event-, construct-, element-specific) do not detect any sequence in the ENA plants contigs dataset (emblconexp bars in Figure 1 A), where GM-sequences are not expected to be present.
GM-specific methods (i.e. event-, construct-, element-specific) do not detect any sequence in the ENA plants contigs dataset (emblconexp bars in Figure 1A), where GM-sequences are not expected to be present.
Among the limitations, it should be pointed out that since this approach is based on the detection of non-GM sequences in a bulk sample it can not account for the hemi- or homozygous state of individual seeds.
Variants of this full-length form (383 amino acid residues) were generated by shifting the BamHI-restricted PCR primer further 5' in the gM coding sequence.
The gM coding sequence without its stop codon (genomic co-ordinates 56950 55796, 383 amino acids) [9] was cloned as a BamHI/XhoI-restricted PCR product into the BamHI/XhoI sites of pEGFP-N3 (Clontech, Palo Alto, CA).
On the GM-CSF blot, the GM-CSF sequence was detected in all three classes of mRNA from cells transfected with GEO-D03 consistent with the predicted splicing pattern (Fig. 2A), in which the gm-csf sequence is present in all classes of mRNA.
The gm-csf sequence was not detected in RNA from cells transfected with JS7 DNA (Fig. 2B).
Migration patterns are similar from both plasmids; therefore, addition of the GM-CSF sequence to the JS7 plasmid did not affect the ability to express full-length Gag and Env proteins.
