The Cm marker was replaced by a gentamicin (Gm) marker.
The Gm marker of pJNNmcs (previously known as pTN-1 (Wierckx et al. 2005)) was obtained by PCR using primers KN34 and KN30 (Table 2) and cloned into the PagI and NcoI restriction sites of pBNTmcs(Cmr), yielding pBNTmcs(Gmr).
Allotypes expressed on the constant region of IgG heavy chain are designated as Gm (Genetic markers) together with the subclass.
Furthermore, the caveolae markers GM-1 and caveolin-1 [7]–[9] cluster around the phagosome during uptake of virulent lines of L. i. chagasi and continue to co-localize with the parasites for up to 24 h.
Also, green fluorescent protein (GFP -tagged zebrafish Ubiad1 expressed in human ECs localizes in the same perinuclear reGFP -taggedtment and colocalizebrafishthe Golgi markers, GM-130 and γ-adaptin.
The cholesteatomatous suppurative otitis media is a chronic inflammatory process and numerous studies described the involvement of different inflammatory mediators (EGF, TGF-alpha, beta, IL-6, IL-1, and GM-CSF), cell surface markers, and adhesion molecules (1,6 integrin, ICAM ELAM) in the pathomechanism of the disease.
Moreover, expression analysis of HSPC TFs (such as HoxA9, Tal1, Scl, Lmo2) and myelomonocytic markers (such as GM-Csfr, M-Csfr and G-Csfr) paralleled those described by Cozzio et al [6] (see Figure S10 in Supporting Information S1).
The M1 and M2b markers G-CSF, GM-CSF, IL-1β, and IL-1α were also not increased by IL-4, and induced by LPS at 12, 36, and 72 h, proving useful to discriminate between phenotypes at later than 4 h of stimulation.
