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The recovery test showed satisfactory results of 94.6 ± 5.1 105.4 ± 4.9% (n = 5) when the biosensor was used for the determination of genetically modified (GM) DNA sequences extracted from GM soybean samples.

It made it possible to assess the interaction between the factors involved in the process leading to GM DNA quantification.

Wizard-based DNA extraction showed a significant overestimation of the % GM DNA concentration, particularly in the biscuit matrix (see Figure 5).

One typical goal was to compare the means of the response variables (DNA yield and % GM DNA) for various combinations of the classification variables.

Both the DNA yield and the % GM DNA quantification were processed using SAS procedures (version 9, SAS Inst. Inc., Cary, NC, http://www.sas.com).sas.com

For this reason, the conventional parametric approach was applied in the fully crossed three-way (DNA yield) and two-way (% GM DNA) analyses of variance (Tables 2 and 3, respectively).

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Similar(51)

The biomolecular computational assay exhibits detection capability of genetically modified DNA below sub-nanomolar level and is found interference-free by abundant coexistence of non-GM DNA.

DNA plasmids encoding the H1N1/New Caledonia hemagglutinin (HA) gene alone (2.0 µg) (Figure 1A) or co-formulated with rhesus macaque GM-CSF DNA (1.8 µg HA DNA vaccine +0.2 µg GM-CSF) (Figure 1B) were precipitated onto 1.0 mg of 1 3 µM gold particles.

Furthermore, co-delivery of the GM-CSF DNA altered the functionality of HA-specific T cells but this effect markedly differed between the two compartments.

In addition, although both the GM-CSF-adjuvanted and unadjuvanted groups developed strong HA-specific T cell proliferative responses after the first boost, co-delivery of GM-CSF DNA notably enhanced the LPR in the blood.

For both the lung and blood, co-delivery of GM-CSF DNA with the HA DNA vaccine augmented expression of the predominant cytokine (TNF-α in the lung, IFN-γ in the blood) in the CD4+ T cell response.

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