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Left Panel: cell growth data; Right Panel: glucose (circle), xylose (square), and ethanol (triangle) data.
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Black square, glucose; black circle, DCW; black star, total lipids; black up-pointing triangle, ARA yield.
White square and dotted line, abnormal batch without sorbitol; black square and dashed line, sorbitol co-fed with glucose; black circle and solid line, 15 g/L sorbitol added in the initial medium; arrow, glucose fed.
The reproducibility of the whole experimental design (cell growth, cellulase production in fed-flask protocol, analyses) was verified on the central point of the mixture design with lactose inducer (fed-batch containing 1/3 lactose + 1/3 xylose + 1/3 glucose, black circle Figure 1), by repeating this condition 10 times (3 or 4 times in 3 different series).
Growth (black triangles), glucose (white circles), galactose (black squares), lactose (black circles), lactate (white squares) and ethanol (white triangles).
Figure 4B shows one example of depolarization caused by glucose (black circles) at substrate concentrations that range from 0.1 to 5 mM in a representative oocyte.
(C ) Histograms depicting experimentally measured single cell peroxisome abundance distributions for haploid cells grown in 2% glucose (red circles) and haploid cells grown in 0.2% oleic acid (dark red triangles).
The residual D-glucose (open circles), residual D-xylose (solid circles), ethanol (solid squares), and glycerol (open triangles) were measured in g/l. Figure S6.
Xylose (cross), acetate (open diamond), glucose (triangle), ethanol (circle) and xylitol (dashed line) concentrations using suspended S. stipitis (A), immobilized S. stipitis (B), suspended S. stipitis with pH control at 5.5 (C), and immobilized S. stipitis with pH control at 5.5 (D), in 100% bdHSSL.
(a) Effect of in vitro E2 treatment alone (10−8 M) on blood glucose after PIT (blue circle, vehicle; green circle, E2).
Closed circles glucose; open triangles cellobiose.
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