Their global readout of gene use gives a detailed picture of expressed transcription factors, growth factors, and receptors.
While analysis of HGF/c-Met coexpression provides useful insight into the role of ligand-dependent c-Met activation, measuring c-Met phosphorylation would also take into account ligand-independent c-Met activation, theoretically giving a more global readout of c-Met signalling.
We conducted RNA-seq on control and HP1E-depleted testes, reasoning that loss of heterochromatin organizing protein would uniquely perturb the global transcriptional readout from this specialized genome compartment.
Overall, these findings imply the global transcriptional readout in the transfected C2C12 cells described by the microarray platform can be attributed to the impact of the transfection of the Rnf14 variants.
However, we demonstrate that IL-2 accumulation by T cells scales as a power law with antigen quantity, independently of population size, providing a shared quantitative readout of the global antigen load.
This limitation prompted us to seek a way to monitor enduring changes in neurosecretion as readouts of different global states.
As the probes are not localised to any intracellular domains [41], the readout reflects changes in "global" cAMP concentrations and this is appropriate as we show that moexipril has activity against multiple PDE4 isoforms (Fig. 3) that are known to target, via unique N-terminal sequences, multiple and distinct cellular locations [9,10].
More recently, we showed that specific recognition of DNA by E2C implies a coupling among histidine protonation, global structural cooperativity, and dynamics, through an "indirect readout" effect on the protein side (52).
An analysis of global gene expression would, therefore, be a reasonable first readout of any possible changes in cell physiology.
The dynamic ROM observed in freely moving rats in our model of knee joint arthritis might serve as a parameter for global disease activity and might thus represent a promising readout parameter for preclinical assessment regarding the overall efficacy not only of antiarthritic but also of antinociceptive compounds.
Employing these new technologies, the United States has fielded strategic-range UAVs, using communications satellites to relay control signals and sensor readouts between UAVs and control centres over global distances.
