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We note that the global locomotion states that we report are distinct from the cGMP-dependent short intervals of continuous roaming or dwelling behavior, previously described in adults (Fujiwara et al., 2002).
Outside of lethargus, crh-1 mutants exhibited more poorly-defined global locomotion states, although a clear active wakefulness state was observed in 4 out of 16 animals 5 6 hr prior to L4leth.
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The spring mass model is a valid fundament to understand global dynamics of fast legged locomotion under gravity.
However, we would like to stress that this extended version of the Gravity Hypothesis, also considering bridging locomotion, supports the more global explanation that in general gravity is an important factor to explain the evolution of extreme SSD.
Traditionally, planetary rovers use wheels for locomotion.
These behavioral alterations point to defects beyond the locomotion system and may reflect a more global function of CHN-1, as expected for a CHIP homolog in a higher eukaryote32.
Thus, despite the fact that at any given point in time forward locomotion and dwelling are mutually exclusive, distinct global states of wakefulness were not guaranteed a priori.
We noted that our usage of the terms "forward-dominated state" and "dwelling-dominated state" to describe global states caused confusion with the concepts of "forward locomotion" and "dwelling".
Neuropsychomotor assessment using standardized batteries [ 23] revealed impairments and delays for global motor development, visuo-manual prehension coordination, posturo-motor and locomotion acquisitions (static and dynamic balance).
However, our finding that locomotion shapes spatial selectivity makes it unlikely to operate through a global quantity.
Previous work on glycinergic contributions to zebrafish locomotion has shown that mutants and pharmacological blocks of glycine receptors and transporters cause locomotor defects, although these global methods are not able to target specific regions of the brain and spinal cord33,34,35,36,37.
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