Contrary to the relatively well-understood dynamics of the GFP expression system, regulation of MetE generates a feedback loop between global cell physiology and gene expression, whose dynamics is much harder to describe.
The unloading modulus then falls with strain due to the localised cell-wall yielding or failure in the pre-collapse stage and the progressive cell crushing in the plateau stage, before rising sharply during the densification stage which is associated with global cell crushing and foam compaction.
Our quantitative models show how cdr2p can convey information about global cell area and deliver it in the form of a local (nodal) concentration.
Quantitative shape analyses of genome-wide mutant libraries promise to identify mutants exhibiting a wide range of shapes and sizes, and comparisons of genome-wide phenotype databases will provide insight to link shape with global cell physiology and fitness.
The results thus demonstrated the power of the GFA in delineating the dynamic alterations of the glycosylation fluxes by local (enzyme-specific) and global (cell specific productivity) factors.
In chemostat culture, 2,4-D removal efficiency was higher than 97% and global cell growth yields were lesser than those obtained in batch.
Next, we integrated in this basic model the capacity of bistable phenotypic switch in individual cells that depends on the sensing the local and global cell density through the consumption of a diffusion-limited substance in the environment.
To be a good spy, carry a small weapon (ex: pencil/pen), small purse, global cell phone, extra cash, and appropriate identification.
By considering that proteins play their action not alone but into the context of a network of interactions [ 2], the mapping of the interrelationships among proteins is an important step to understand their functions and the global cell behavior [ 3].
In C. crescentus, CtrA is a global cell cycle regulator [ 26] and more specifically, the response regulator protein of a two-component signal transduction signal.
