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Using a glass probe to apply pressure to the cell membrane, we have identified three classes of mechanically activated currents that differ in their kinetics, magnitude and distribution.
For the first 38 days of the experiment (from 26/11/08 to 4/1/09), pH was measured manually twice daily using a pH electrode (WTW Multi 340i with sensorex glass probe).
The cantilever was equipped with a smooth and aligned glass probe.
The sample was approached, formed contact with the glass probe, and was loaded.
Additionally, RBCs were also too small and fragile for indentation using a glass probe with or without simultaneous electrophysiological recording.
Cells were subjected to a series of mechanical stimuli, by pressing a glass probe briefly onto the cell surface for 150 ms (arrows).
Similar(43)
This semi-quantitative method with high sensitivity and spatial resolution has occasionally been employed for CGRP release measurements but is limited by reliable fixation of CGRP antibodies to the glass probes [34].
Briefly, stiff glass probes were fire polished and shaped to fit into the concave aspect of the outer hair cell stereocilia.
With the surgical microscope and glass probes, this cavity was carefully inspected visually to confirm the absence of spared spinal tissue, and then filled with Gelfoam.
Subsequently, the forward primer was modified with -NH2 at 5' end, and then fixed on the aldehyde coating glass as probe leaving 3'-OH free.
A drop of mineral oil was placed in the cavity to aid optical connectivity and the glass optical probe was placed into the cavity and fixed using Loctite superglue (Henkel, UK).
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