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Glass knife and ultramicrotome were used to cut the samples into 1 μM and samples placed onto glass slides, stained with toluidine blue, dried, washed the stain, and examined under light microscope.
Glass, knife, cloth.
Ultrathin sections of the pelleted samples were cut on an RMC MT-X ultra-microtome using a glass knife.
The prepared longitudinally and transversely cut segments of biological tissues were trimmed using a Reichert UM-03 ultramicrotome glass knife (Puzyr' et al. 1998; Floriani 2005).
Sections of 1 μm were obtained by a glass knife on an ultramicrotome (Leica, Wetzlar, Germany), and were stained with 0.1% alkaline TBO for 1 min at 60°C on a hot plate.
Small (1 mm × 1 mm × 0.5 mm) pieces of resin-embedded tissues were mounted on aluminum specimen pins (Gatan, Inc., Pleasanton, CA, USA) using cyanoacrylic glue and precision trimmed with a glass knife to a rectangle approximately 0.5 mm × 1.0 mm, so that tissue was exposed on all four sides.
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The lawyers said that Mr. Komisarjevsky was sexually abused as a child, suffered mood disorders and head injuries, abused drugs and cut himself with glass, knives and razors, and that his evangelical Christian adoptive parents denied him proper care, relying instead on religion.
Inside a room that is roughly 10 feet long and 7 feet wide, Ulf Dahl spends up to two days each week hunched over a microscope with his remarkably steady fingertips and the tiny glass knives he makes over a hot flame.
Then, 3 µm-thick sections were cut with glass knives using a Reichert-Jung 2040 Autocut rotary microtome.
Freshly sharpened glass knives with cutting edge of 45° were used to get the cryosections of 50 70 nm thickness.
Serial, 3 µm-thick sections were cut with glass knives using a Reichert-Jung 2040 Autocut rotary microtome.
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