Sentence examples for glacial acid from inspiring English sources
noun
Anhydrous acetic acid
First, a cell count is performed on the specimen to establish a plating volume and based on the cell count, a corresponding volume of bone marrow is added to 2 culture flasks containing culture medium and incubated for 24 to 48 h at 37 degrees C. In the harvest process, the cells are exposed to colcemid and hypotonic solution, and are fixed with glacial acid and methanol.
Ethylene diamine tetraacetic acid (EDTA), sulphuric acid (H2SO4,), barium chloride (BaCl2), hydrochloric acid (HCl) sodium hydroxide (NaOH), sodium fluoride (NaF) and glacial acid acetic (CH3COOH) are used to analyze the real water.
The reaction was stopped in 3% glacial acid.
Filtered sample was analyzed using mobile phase consisting acetonitryl and methanol (45:55) (HPLC grade) with 1 ml glacial acid and 0.5 ml sodium acetate [ 26, 32].
Acid formalin (AF), composed by 10%% formalin diluted with 70%% ethanol and 5%% glacial acid (all Carlo Erba Reagenti SpA, Milan, Italy) was here introduced for its capacity to induce HA solidification [ 19].
The mobile phase consisted of a mixture of a solution of sodium acetate (pH 4.7; 0.1 M) and acetonitrile 60 40 (v/v), with pH adjusted to 5 by addition of acetic glacial acid.
After applying the sample to a glass slide and drying it in the air, fixation was performed by a cold methanol-glacial acid mix (3 : 1) for 30 min.
Samples filtered and two ml of the filtrate was mixed with 3 mL of ninhydrin reagent ( 1.25 g ninhydrin in 30 mL glacial acetic acid and 20 mL 6 M phosphoric acid) and 2 mL of glacial acetic acid.
1. Prepare the following solvents separately: (a) acetonitrile with 0.001% glacial acetic acid, (b) water with 0.001% glacial acetic acid, (c) methanol with 0.001% glacial acetic acid.
Glacial acetic acid, HPLC grade acetonitrile, sodium hydroxide, hydrochloric acid and hydrogen peroxide were obtained from Merck Ltd., Mumbai, India.
The gels were destained with destaining solution (40% methanol, 10% glacial acetic acid), stored in 5% glacial acetic acid.
