In the second step, both ANN and ANFIS models were calibrated and verified using GL data of each piezometer, rainfall and runoff considering various input scenarios to predict the GL at one month ahead.
This was seen for all deficiencies of 45C examined [ Df 2R Np3, Df 2R Np5, and Df 2R wun GL ; data not shown].
We also investigated whether JNK signaling affects GL-mediated ATF3 promoter activation and found that JNK inhibition by SP600125 did not affect ATF3 promoter activation by GL (data not shown).
This was corroborated by immunoprecipitation, where strong bands were identified with anti-gB and anti-gD (Fig. 2E) but not with anti-gH or anti-gL (data not shown).
For all univariate analyses on CE and GL, the data from this source for a stillborn calf was set as missing but the remaining data obtained by the specific data source was used.
GL organised data and constructed the graphs.
GL analysed data and drafted the manuscript in collaboration with JS.
GL: conception, design, data collection, data analysis, manuscript preparation BR, MP, DL, BT: design, data collection, manuscript preparation The pre-publication history for this paper can be accessed here: Represents EMG onset times relative to hamstring firing.
P-GL, collection of data, P-GL analysis of air-dried smears and P-GL, BS-P, KRR and SL contributed to interpretation of results, statistical analysis and writing of the study report.
KW: RG, YG, JB and GL analyzed the data and advised on data analysis.
Study concept and design, MK and OG; acquisition of data, GL, KML, LT, JV, AA, and MK; analysis and interpretation of data, MK, GL, and OG; drafting of the manuscript, MK and OG; critical revision of the manuscript for important intellectual content, LB and JR; statistical analysis, AH, GL, and MK.
