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PKINES-Flip1 has the inverted wild-type LDI at the N-terminus, giving sequence I D L NE LALK LAG L. The two hydrophobic side chains in the N-terminal Φ-X-Φ were incrementally made larger to generate PKINES-Flip2 (FDLNELALKLAGL) and PKINES-Flip3 (FDMNELALKLAGL) mutants.
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