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In our method, a set of sections covering five grids satisfy the needs of both qualitative and quantitative observations, giving a good overview of autophagic events at all developmental stages, and in all major cell types of the body (Sigmond et al. 2008).
Although each site was only visited once, nearby sites were sampled during every period and year giving a good overview of mosquito presence in a specific area.
This range of SNR ratios was chosen, as they vary from being very gentle to very aggressive, giving a good overview of the trends.
In addition, while the specific and limited range of responses allowable assisted in giving a good overview of quantifiable data, further qualitative research would likely be of benefit to explore further patient views and level of understanding.
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