It shows that the washing treatments (in sect. "Experimental section") given to the samples for the heavy metals removal are successful.
The Rasch item separation index for the VELC Test® was 31.07, indicating that when given to the sample, item difficulty estimates spread out along the logits scale and could be split into 31 strata.
For samples y1,..,yh, simulate grades g1,..,gh from the observed empirical distributions of grades given to the sample (i.e. for a given sample, with probability of selecting grade j proportional to the proportion of raters who assigned grade j to the sample).
Of note, meaningful measurements are dependent upon careful thought given to the sampling process.
Consideration must also be given to the sample sizes achieved for each site and team type.
A code number different from the one assigned in the first round was given to the samples.
To obscure the sample identity from the labs, a different code number from the one assigned in the second round was given to the samples.
An aliquot of approximately 0.6 g was weighed and handled like the liquids above, whereas the acetonitrile/isopropanol solution was given to the sample mixture for protein precipitation.
At the time of insertion, NGS-Logistics automatically assigns an internal Sample ID to each sample to pseudonymize the actual name (Sample Local Name) given to the sample by the owner from the rest of the users.
Detailed consideration should be given to the sample selection, inclusion and exclusion criteria, treatment (if possible randomised or standardised), the use of standardised assays, and statistical analysis (see later).
