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Leaf samples were harvested at given time points after treatments for total RNA extraction.
We therefore analyzed the toxicity at the given time points through standard histology of the pancreas, lungs, bowel, and kidneys.
*p < 0.05, **p < 0.01, and ***p < 0.001 for comparisons between pre- and post-race values of all runners at given time points.
Constructs were grown in PD98059 supplemented medium for the given time points.
Cells were trypsinized and analyzed by Coulter counting at given time points.
At the given time points, cells were diluted in sterile 150 mM NaCl and plated for colony forming units.
Cells were cultured and supernatants were collected after given time points and stored at -20°C until cytokine determination.
At given time points, viable embryos (defined by embryonic movement at candling) were humanely sacrificed by chilling the eggs on ice for 30 60 min. The eggs' surface was then disinfected with 70% ethanol.
At given time points, 50 µl aliquots from the lower chamber were removed for measurement and replaced with 50 µl of fresh medium in order to maintain the hydrostatic equilibrium.
A431 cells were treated in serum free medium for the given time points and then the media was removed and spun at 2000 g for 10 min to pellet down cells, if any.
We monitored CD25 and FOXP3 expression by flow cytometry and measured the methylation status of the FOXP3 promoter at given time points (day 0, 7, 14 and 21) using the COBRA based method previously described.
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